Offshore Scotian Shelf biodiversity assessment using eDNA metabarcoding 2025

This resource contains eDNA metabarcoding derived species records from two different markers - 12S to target marine fishes (the MiFish-U marker) and COI to target invertebrates and general biodiversity. Samples were collected in summer 2025 (July and August) as part of the annual DFO multispecies trawl survey, and in October 2025 as part of the annual Atlantic Zone Monitoring Program. Seawater samples were collected from near bottom using a CTD rosette and filtered aboard the research vessel using a Smith Root vacuum pump and 1.2 um filters. eDNA was extracted from the filters and sequenced on an Illumina MiSeq platform at the Aquatic Biotechnology Laboratory based at the Bedford Institute of Oceanography. Raw sequence reads were demultiplexed and denoised using DADA2 in the QIIME2 pipeline, and taxonomy was assigned to amplicon sequence variants using BLASTn and the NCBI eukaryote nucleotide database. 

Citation: Stanley R, Jeffery N (2026). Offshore Scotian Shelf biodiversity assessment using eDNA metabarcoding 2025. Version 1.1. Fisheries and Oceans Canada. Occurrence dataset. https://ipt.iobis.org/obiscanada/resource?r=dfo_maritimes_offshore_edna_2025&v=1.1

Published: May 07, 2026 at 15:31

First registration: May 07, 2026 at 14:02

License: This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License

URL: https://ipt.iobis.org/obiscanada/resource?r=dfo_maritimes_offshore_edna_2025