Metabarcoding of Benthic Marine Communities in Nova Scotia and New Brunswick

URLhttps://ipt.iobis.org/obiscanada/resource?r=metabarcoding_benthic_marine_communities_in_novascotia_newbrunswick_2021
Repository URL http://ipt.vliz.be/obiscanada/
Node OBIS Canada
Published2025-02-28 15:29
First registered2025-03-08 22:45
Abstract

To monitor marine invasive species distribution and broad taxonomic diversity in the subtidal fauna and flora of the DFO Maritimes region, metabarcoding of eDNA samples was performed at a total of 49 sites between 2021-2022. For each site, three 1-L amber bottles of seawater collected from ~1m above the benthos at each site was filtered with Sterivex 0.22-µm filters prior to DNA extraction with a Qiagen DNEasy Power Water Kit and sequencing with an Illumina NovaSeqtm6000 instrument (primers are included in metadata). Raw paired-end reads were merged with NGmerge v0.3 and denoised with DnoisE v1.4.1 (DnoisE settings are included in the metadata). Singletons were removed and remaining sequences were assigned to species-level by BLAST against a local copy of the nr/nt database (downloaded Nov 2023).

CitationKrumhansl K, Brooks C, Kingsbury S, DiBacco C (2025). Metabarcoding of Benthic Marine Communities in Nova Scotia and New Brunswick. Version 1.1. Fisheries and Oceans Canada. Occurrence dataset. https://ipt.iobis.org/obiscanada/resource?r=metabarcoding_benthic_marine_communities_in_novascotia_newbrunswick_2021&v=1.1
RightsThis work is licensed under a Creative Commons Attribution (CC-BY 4.0) License
Keywords Occurrence, eDNA, metabarcoding, invasive species, benthic marine communities
Contacts
Creator Kira Krumhansl
Fisheries and Oceans Canada
Contact Kira Krumhansl
Fisheries and Oceans Canada
Metadata Provider Kira Krumhansl
Fisheries and Oceans Canada
Metadata Provider Cody Brooks
Fisheries and Oceans Canada
Metadata Provider Sarah Kingsbury
Fisheries and Oceans Canada
Metadata Provider Claudio DiBacco
Fisheries and Oceans Canada

Statistics

Occurrence records 236,304
Species level 166,751
Absence records 0
Event records 0
Species 2,407
Taxa 3,173
Time range 2021 - 2022

This dataset has appeared in 198 downloads in 2025, with a total of 11,393,535 records.

Data quality

Dropped records

Dropped records 347,636
Not marine 2,999
No WoRMS match 344,081
No coordinates 2,802
Zero coordinates 0

Taxonomic issues

Marine unsure 18,488
No accepted name available 21,431

Missing and invalid fields

Field Missing Invalid
coordinateUncertaintyInMeters 583,940 0
decimalLatitude 0 2,802
decimalLongitude 0 2,802
maximumDepthInMeters 583,940 0
minimumDepthInMeters 583,940 0
scientificNameID 0 583,940

Spatial issues

Records on land 78,683
More than 20 km from shore 0
Depth exceeds bathymetric depth 0

Top taxa

IUCN Red List All taxa
Scientific name Records
Bathycoccus prasinos Eikrem & Throndsen, 1990
Phylum Chlorophyta > Class Mamiellophyceae
22,990
Micromonas pusilla (Butcher) Manton & Parke, 1960
Phylum Chlorophyta > Class Mamiellophyceae
16,430
Picochlorum W.J.Henley, J.L.Hironaka, L.Guillou, M.A.Buchheim, J.A.Buchheim, M.W.Fawley & K.P.Fawley, 2004
Phylum Chlorophyta > Class Trebouxiophyceae
9,553
Nannochloris Naumann, 1921
Phylum Chlorophyta > Class Trebouxiophyceae
5,113
Amoebophrya Koeppen, 1894
Phylum Myzozoa > Class Dinophyceae
4,736
Bellerochea polymorpha Hargraves & Guillard, 1974
Phylum Heterokontophyta > Class Bacillariophyceae
3,995
Heterocapsa rotundata (Lohmann) Gert Hansen, 1995
Phylum Myzozoa > Class Dinophyceae
3,772
Thalassiosira P.T. Cleve, 1873 emend. Hasle, 1973
Phylum Heterokontophyta > Class Bacillariophyceae
3,712
Mediolabrus comicus (H.Takano) Yang Li, 2020
Phylum Heterokontophyta > Class Bacillariophyceae
3,660
Tripos muelleri Bory de Saint-Vincent, 1826
Phylum Myzozoa > Class Dinophyceae
3,642

Distribution

Records

Composition

detailed (class) simple

DNA barcodes

Barcode region Occurrence records
18S 130,432
COI 105,872

Occurrences

scientificNameeventDatedecimalLongitudedecimalLatitudecollectionCodecatalogNumber