Name: Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels.
License: This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY) 4.0 License

Methods evaluation for sample collection and nucleic acid extraction from multiple trophic levels simultaneously (i.e. bacteria, phytoplankton, zooplankton, vertebrates). Specifically, filter types and DNA extraction kits were evaluated for producing Illumina MiSeq reads for 12S, 16S and 18S amplicons from water samples collected in Monterey Bay California.

Published: September 20, 2022 at 12:42

License: This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY) 4.0 License

URL: https://hosted-datasets.gbif.org/mgnify/MGYS00004176.zip

Contacts:

Monterey Bay Aquarium Research Institution
Monterey Bay Aquarium Research Institution

2,757

occurrence records

1,596

taxa

245

species

Taxa

Missing and invalid fields

Field	Missing
coordinateUncertaintyInMeters	2,757	100.0%
occurrenceStatus	2,757	100.0%
scientificNameID	2,757	100.0%

Quality flags

The OBIS data quality flags are documented at https://github.com/iobis/obis-qc.

Flag	Records
NO_MATCH	821	29.8%
MARINE_UNSURE	195	7.1%
NO_ACCEPTED_NAME	27	1.0%
NOT_MARINE	4	0.1%
WORMS_ANNOTATION_REJECT_AMBIGUOUS	2	0.1%
WORMS_ANNOTATION_UNRESOLVABLE	2	0.1%
WORMS_ANNOTATION_RESOLVABLE	1	0.0%

Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels.

Taxa

Missing and invalid fields

Quality flags

Measurement types

DNA derived data