Name: Incubation of marine methane seep sediments with a bioorthogonal amino acid
License: This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY) 4.0 License

In marine sediments, anaerobic oxidation of methane (AOM) is primarily catalyzed by syntrophic consortia of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB). We applied bioorthogonal non-canonical amino acid tagging (BONCAT) using a high-throughput approach to detecting protein synthesis in individual cells, on sediments from deep-sea methane seeps. Sediment samples have been incubated in the presence or absence of methane and a bioorthogonal amino acid. V6 regions of bacterial and archaeal 16S rRNA genes were amplified using general primers, tagged, and analyzed for shifts in the microbial community during time of incubation.

Published: September 20, 2022 at 12:42

License: This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY) 4.0 License

URL: https://hosted-datasets.gbif.org/mgnify/MGYS00002843.zip

Contacts:

California Institute of Technology
California Institute of Technology

15,325

occurrence records

659

taxa

species

Taxa

Missing and invalid fields

Field	Missing
coordinateUncertaintyInMeters	15,325	100.0%
maximumDepthInMeters	15,325	100.0%
minimumDepthInMeters	15,325	100.0%
occurrenceStatus	15,325	100.0%
scientificNameID	15,325	100.0%

Quality flags

The OBIS data quality flags are documented at https://github.com/iobis/obis-qc.

Flag	Records
NO_DEPTH	15,325	100.0%
NO_MATCH	6,613	43.2%
MARINE_UNSURE	1,908	12.5%
NOT_MARINE	62	0.4%
NO_ACCEPTED_NAME	13	0.1%

Incubation of marine methane seep sediments with a bioorthogonal amino acid

Taxa

Missing and invalid fields

Quality flags

Measurement types

DNA derived data